Journal: Arthritis Research & Therapy

Article Title: Divergent roles of prostacyclin and PGE 2 in human tendinopathy

doi: 10.1186/s13075-019-1855-5

Figure Lengend Snippet: List of primary antibodies used. Antibody for mPGES1, in-house [ 54 ]

Article Snippet: CD34 , Proteintech, cat# 60180 , IgG 2b , mouse , 1:100.

Techniques:

Journal: Arthritis Research & Therapy

Article Title: Divergent roles of prostacyclin and PGE 2 in human tendinopathy

doi: 10.1186/s13075-019-1855-5

Figure Lengend Snippet: Expression of fibroblast activation marker Podoplanin (PDPN) and nociceptive neuromodulator NMDAR-1 in diseased supraspinatus tendon tissues. a – b Representative immunofluorescence images showing staining of cell nuclei (POPO-1, cyan) and PDPN (green) with a expression of NMDAR-1 (red) and PGIS (violet) and b expression of TLR4 (red) and IL-1R (violet). c Representative confocal immunofluorescence images showing merged image of diseased tendon sections stained with isotype control antibodies for mouse IgG1, IgG2a, and IgG2b and rabbit IgG fractions. Cyan represents POPO-1 nuclear counterstain. Scale bar, 20 μm

Article Snippet: CD34 , Proteintech, cat# 60180 , IgG 2b , mouse , 1:100.

Techniques: Expressing, Activation Assay, Marker, Immunofluorescence, Staining, Control

Journal: BioMed Research International

Article Title: Chronic Heat Stress Inhibits Immune Responses to H5N1 Vaccination through Regulating CD4 + CD25 + Foxp3 + Tregs

doi: 10.1155/2013/160859

Figure Lengend Snippet: CHS negatively influences the immune effects induced by formalin-inactivated H5N1 virus. Serum samples and T cells of three mice from CHS+Ag and TN+Ag groups were collected on indicated days postprimary immunization. (a) Antibody levels against the H5 subtype antigen were expressed by HI titer. (b) Ag-specific IgG, IgG1, and IgG2a were determined by ELISA. (c) T cells were isolated on day 21 and stimulated with specific antigen; cell proliferation was determined by MTS/PMS method. (d) T cells collected on day 21 were stimulated with a viral-specific antigen for 6 h in vitro , stained with different florescent antibodies, and analyzed by flow cytometry. The numbers in the box indicate the percentages of examined cytokines. Data are summarized in a bar graph to the right. Results shown are pooled from three independent experiments.

Article Snippet: After blocking, plates were incubated with the mice serum samples in a serial dilution at 37°C for 1 h. Then, a 1 : 2000 dilution HRP-conjugated goat anti-mouse IgG, IgG1, and IgG2a (Proteintech, USA) were added.

Techniques: Virus, Enzyme-linked Immunosorbent Assay, Isolation, In Vitro, Staining, Flow Cytometry